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6. PROCEDURE NOTES
• The thick film should be dried flat and be protected from dust and flies.
• The thick film may autofixate if exposed to extreme heat and should therefore be stained
immediately.
• The thick film can be dried gently with a hair-dryer set at warm or another drying method, but care
must be taken to avoid heat fixation, which can occur quickly. Issue a hair-dryer only to technicians
who have demonstrated competence with this method.
• Do not use a ballpoint or gel pen to label slides, as the ink will spread when the film is fixed.
Correctly made slides leave little blood on the spreader slide, which can be used for making thick and thin
slides from the next patient, while another, clean slide from the package is used as a fresh spreader. Do not use
a slide as a spreader more than once
7. REFERENCE
WHO.Basic malaria microscopy.Part I. Learner’s guide.Second edition. Geneva: 2010.
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