Page 107 - Buku Panduan Perkhidmatan MKAKB

MM–SOP–05A: COLLECTION OF FINGER-PRICK BLOOD AND PREPARATION OF THICK AND THIN BLOOD FILMS

FLOW CHART DESCRIPTION OF ACTIVITY ILLUSTRATION

8. Collect two or three more small 8. Apply further gentle pressure
drops of blood, and use them to make to express more blood, and

the thick film. collect two or three drops on
the slide about 1 cm from the

drop intended for the thin film.

9. Wipe the remaining blood

9. Wipe the remaining blood from from the finger with clean,

the finger. dry cotton.

10. Do not pause between

applying and spreading the

drops. Prepare the blood films
10. Place the slide with the blood facing
with the slide lying on a
up on a flat surface.
flat surface.

11. To prepare the thin film,

place the edge of a clean
o
“spreader” slide at 45 in front
of the blood drop intended for
the thin film.

12. Slowly pull the “spreader”
11–13. Using a clean “spreader” slide, back until it touches the
make the thin film by pushing forwards drop of blood and the blood
the one drop of blood in a smooth, spreads along the edge of the

continuous motion. “spreader”.

13. Rapidly push the “spreader”
forwards (away from
the centre) in a smooth,
continuous motion, until the
spreader leaves a “feathery”
end for the

thin film.

14. With the corner of the same
“spreader” used for making
the thin film, make the thick
film by swirling the three

drops of blood together
14. With the corner of the “spreader”,
forming a circle of about 1 cm
make the thick film by swirling the three
in diameter size.
drops of blood together to form a circle.
Do not stir the blood. A

circular or rectangular film

can be made by three to six
quick strokes with the corner

of the spreader.

15. After preparing the thin and
thick blood films, allow them

to dry in air in a horizontal
15. Air-dry in a position on a slide tray. If
horizontal position. A slide dryer rapid drying is required,
may be used if rapid drying dry the films with low heat
is required. from a hair-dryer for 5 s, at
a distance of 30 cm. Do not
place the slides too close to
the dryer, as the films might

become heat fixed.

Buku Panduan MKAKB 72